{"id":543,"date":"2025-11-20T14:48:29","date_gmt":"2025-11-20T13:48:29","guid":{"rendered":"https:\/\/amanitalabs.tech\/?post_type=product&p=543"},"modified":"2025-11-20T14:48:29","modified_gmt":"2025-11-20T13:48:29","slug":"cepa-madre-de-ostra-rey-perla-negra-en-pda","status":"publish","type":"product","link":"https:\/\/amanitalabs.tech\/?product=cepa-madre-de-ostra-rey-perla-negra-en-pda","title":{"rendered":"Cepa madre de Ostra Rey Perla Negra en PDA"},"content":{"rendered":"

Descripci\u00f3n del Producto:<\/span><\/strong>
\nCepa madre estabilizada de Pleurotus ostreatus var. ‘Black Pearl’ en placa Petri con medio PDA. Este aislado de \u00e9lite garantiza gen\u00e9tica selecta para producci\u00f3n comercial, con caracter\u00edsticas superiores de coloraci\u00f3n oscura, alto rendimiento y resistencia.<\/span><\/p>\n

Protocolo de Mantenimiento de Cepa Madre<\/span><\/p>\n

1. Condiciones de Almacenamiento:<\/span><\/strong><\/p>\n

Temperatura: 2-4\u00b0C en refrigeraci\u00f3n<\/span><\/p>\n

Posici\u00f3n: Placas siempre invertidas<\/span><\/p>\n

Vida \u00fatil: 45-60 d\u00edas en refrigeraci\u00f3n<\/span><\/p>\n

Medio \u00f3ptimo: PDA (Patato Dextrosa Agar)<\/span><\/p>\n

2. Transferencia y Reactivaci\u00f3n:<\/span><\/strong><\/p>\n

Frecuencia: Cada 30 d\u00edas como m\u00e1ximo<\/span><\/p>\n

Tama\u00f1o de in\u00f3culo: 5×5 mm del borde de crecimiento activo<\/span><\/p>\n

Temperatura de reactivaci\u00f3n: 22-24\u00b0C<\/span><\/p>\n

Tiempo de recuperaci\u00f3n: 24-48 horas<\/span><\/p>\n

3. Caracter\u00edsticas de Calidad de la Cepa:<\/span><\/strong><\/p>\n

Color: Blanco puro intenso<\/span><\/p>\n

Textura: Micelio rizomorfo denso<\/span><\/p>\n

Crecimiento: Uniforme, sin sectores<\/span><\/p>\n

Velocidad: Colonizaci\u00f3n completa en 5-7 d\u00edas<\/span><\/p>\n

M\u00e9todos de Propagaci\u00f3n desde Cepa Madre<\/span><\/p>\n

Para Producci\u00f3n de Granos:<\/span><\/strong><\/p>\n

Cortar 6-8 cuadrados de 1×1 cm<\/span><\/p>\n

Inocular en frascos de grano est\u00e9ril de 1L<\/span><\/p>\n

1 placa = 5-10 frascos de spawn<\/span><\/p>\n

Para Expansi\u00f3n en Placas:<\/span><\/strong><\/p>\n

-Realizar m\u00e1ximo 5 transferencias desde esta cepa madre<\/span><\/p>\n

-Usar siempre medio PDA fresco<\/span><\/p>\n

-Mantener registro generacional<\/span><\/p>\n

-Para Iniciar Cultivo L\u00edquido:<\/span><\/p>\n

-Transferir 3-4 fragmentos a caldo nutritivo est\u00e9ril<\/span><\/p>\n

-Incubar con agitaci\u00f3n a 22-24\u00b0C<\/span><\/p>\n

Protocolo de Transferencia Est\u00e9ril<\/span><\/strong><\/p>\n

1. Preparaci\u00f3n Previa:<\/span><\/strong><\/p>\n

Desinfectar c\u00e1mara de flujo laminar con UV 30 min<\/span><\/p>\n

Esterilizar pinzas y escalpelo al rojo vivo<\/span><\/p>\n

Pre-calentar nuevas placas a temperatura ambiente<\/span><\/p>\n

2. Proceso de Transferencia:<\/span><\/strong><\/p>\n

Seleccionar sector del borde m\u00e1s vigoroso<\/span><\/p>\n

Cortar cuadrado limpio sin arrastrar agar<\/span><\/p>\n

Depositar en centro de nueva placa<\/span><\/p>\n

Sellar inmediatamente con parafilm<\/span><\/p>\n

3. Control de Calidad Post-Transferencia:<\/span><\/strong><\/p>\n

Verificar crecimiento en primeras 24 horas<\/span><\/p>\n

Descartar placas con contaminaci\u00f3n<\/span><\/p>\n

Documentar caracter\u00edsticas del crecimiento<\/span><\/p>\n

Aplicaciones Espec\u00edficas para Esta Cepa:<\/span><\/p>\n

Producci\u00f3n Comercial:<\/span><\/strong><\/p>\n

In\u00f3culo para bolsas de 3-5 kg de sustrato<\/span><\/p>\n

M\u00e1ximo rendimiento en paja pasteurizada<\/span><\/p>\n

Excelente comportamiento en aserr\u00edn enriquecido<\/span><\/p>\n

Caracter\u00edsticas de Fructificaci\u00f3n:<\/span><\/strong><\/p>\n

Color: Gris oscuro a negro met\u00e1lico<\/span><\/p>\n

Tama\u00f1o: Sombreros 5-12 cm<\/span><\/p>\n

Densidad: Alta carnocidad<\/span><\/p>\n

Se\u00f1ales de Alerta:<\/span><\/strong><\/p>\n

\u274c Crecimiento lento o sectorizado<\/span><\/p>\n

\u274c Cambios de coloraci\u00f3n (amarillo, marr\u00f3n)<\/span><\/p>\n

\u274c Presencia de exudados l\u00edquidos<\/span><\/p>\n

\u274c Olores diferentes al caracter\u00edstico<\/span><\/p>\n

\u2705 Indicadores de Calidad:<\/span><\/strong><\/p>\n

Crecimiento radial uniforme<\/span><\/p>\n

Blanco puro intenso<\/span><\/p>\n

Textura aterciopelada densa<\/span><\/p>\n

Ausencia completa de contaminantes<\/span><\/p>\n

\u00a1Esta cepa madre representa la base gen\u00e9tica para producciones comerciales de alta calidad! Mantener su estabilidad es crucial para resultados consistentes.<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

Pleorotus Ostreatus var. \u00abBlack Pearl King\u00bb<\/p>\n","protected":false},"featured_media":149,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[26],"product_tag":[],"class_list":{"0":"post-543","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-esporas-aisladas","8":"first","9":"instock","10":"shipping-taxable","11":"purchasable","12":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/amanitalabs.tech\/index.php?rest_route=\/wp\/v2\/product\/543","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/amanitalabs.tech\/index.php?rest_route=\/wp\/v2\/product"}],"about":[{"href":"https:\/\/amanitalabs.tech\/index.php?rest_route=\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/amanitalabs.tech\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=543"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/amanitalabs.tech\/index.php?rest_route=\/wp\/v2\/media\/149"}],"wp:attachment":[{"href":"https:\/\/amanitalabs.tech\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=543"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/amanitalabs.tech\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_brand&post=543"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/amanitalabs.tech\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_cat&post=543"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/amanitalabs.tech\/index.php?rest_route=%2Fwp%2Fv2%2Fproduct_tag&post=543"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}